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12. Add 500 µl Buffer RPE to the RNeasy MinElute spin column. Close the lid gently and centrifuge for 2 min at ≥8000 x g (≥10000 rpm) to wash the spin column. After centrifugation carefully remove the spin column from the collection tube so that the column does not contact the flow-through. 13. Bone powders are digested with proteinase K, and the DNA is purified directly using silica‐based spin columns (QIAquick™, QIAGEN). The efficiency of this protocol is demonstrated using human bone samples ranging in age from 15 to 5,000 years old.

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